Biodiesel Byproduct and Biofuel Synthesizing Clostridium acetobutylicum

  • James F. Graves University of Detroit Mercy
Keywords: Biodiesel, Glycerol, Clostridium acetobutylicum, Butanol


In the manufacture of biodiesel, glycerol is a byproduct.  Acetone-butanol-ethanol (ABE) fermentation by anaerobe Clostridium acetobutylicum ia also a source of biofuel.  Growth of C. acetobutylicum in the presence of waste biodiesel glycerol byproduct was examined in this investigation.  In cultures containing added glucose or a mixture of glucose and byproduct the optical density (OD) was increased and a positive fermentation reaction was exhibited while results in cultures containing byproduct or pure glycerol were similar to those in the base medium.  The pH of cultures containing glucose markedly decreased. The concentration of glucose dropped to an undetectable level.  All cultures were determined to contain alcohol.  The potential applications for biodiesel glycerol byproduct need to e explored.


Monthly PDF Downloads

Download data is not yet available.

Author Biography

James F. Graves, University of Detroit Mercy


Department of Biology

Associate Professor


How to Cite
Graves, J. F. (2017). Biodiesel Byproduct and Biofuel Synthesizing Clostridium acetobutylicum. Journal of Student Research, 6(1), 109-111. Retrieved from
Research Articles